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Rabbit Anti-His tag  antibody (bs-0287R)  
~~~促銷代碼KT202411~~~
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產品編號 bs-0287R
英文名稱 Rabbit Anti-His tag  antibody
中文名稱 His tag標簽抗體
別    名 HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.  
Specific References  (17)     |     bs-0287R has been referenced in 17 publications.
[IF=2.914]   WB ;  Pig.  
[IF=19.924] Yifan Yang. et al. A Versatile Platform for the Tumor-Targeted Intracellular Delivery of Peptides, Proteins, and siRNA. ADV FUNCT MATER. 2023 Apr;:2301011  WB ;  Human.  
[IF=7.2] Zhiyan Hu. et al. CIP4 targeted to recruit GTP-Cdc42 involving in invadopodia formation via NF-κB signaling pathway promotes invasion and metastasis of CRC. Mol Ther-Oncolytics. 2022 Feb;:  WB ;  Strain（Bl21）.  
[IF=4.38] Ma, Xiangyi, et al. "DT390-triTMTP1, a novel fusion protein of diphtheria toxin with tandem repeat TMTP1 peptide, preferentially targets metastatic tumors." Molecular pharmaceutics 10.1 (2013): 115-126.  IHC-P ;  
[IF=4.235] Xu Zhenshang. et al. Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases. Front Microbiol. 2020 Nov;11:2281  WB ;  
[IF=3.309] Chuandong Cheng. et al. SREBP2/Rab11s/GLUT1/6 network regulates proliferation and migration of glioblastoma. PATHOL RES PRACT. 2022 Oct;:154176  WB ;  Human.  
[IF=2.984] Ruirui Luoet al. Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines. Gene . 2020 Oct 30;759:144999.  WB ;  pig.  
[IF=2.984]   WB, IF ;  Pig.  
[IF=2.914] Xiaoli Gao. et al. Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathogenesis. 2020 Oct;147:104379  WB ;  Pig.  
[IF=2.913] Li N et al. Identification and characterization of an atypical RIG-I encoded by planarian Dugesia japonica and its essential role in the immune response.(2019) Dev Comp Immunol. Feb;91:72-84.  WB ;  
[IF=2.7] Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013).  WB ;  
[IF=2.7] WANG, Peng, et al. "Construction and identification of a recombinant adenovirus vector expressing His-tag-ICP47 fusion gene." Life Science Journal 9.1 (2012).  
[IF=2.56] Wang, P. et al. "Attenuation of cytokine production of interleukine -2 and perforin in Two-way mixed lymphocyte reaction by highly efficient transduction of the recombinant adenovirus expressing ICP47 gene."Life Sci J 2013;10(3):963-969  WB ;  
[IF=1.74] Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013).  WB ;  
[IF=1.676] Ma et al. Adjuvant effects mediated by the carbohydrate recognition domain of Agrocybe aegerita lectin interacting with avian influenza H9N2 viral surface glycosylated proteins. (2017) J.Zhejiang.Univ.Sci.. 18:653-661  other ;  
[IF=1.109] He,et al.Recombinant Fiber-2 Protein-Based Indirect ELISA for Antibody Detection of Fowl Adenovirus Serotype 4.(2018) Avian Diseases. 62:73-78.  WB ;  
[IF=0] Ren et al. Long-term rescue of rat retinal ganglion cells and visual function by AAV-mediated BDNF expression after acute elevation of intraocular pressure. (2012) Invest.Ophthalmol.Vis.Sci. 53:1003-11  IHC ;  
產品類型 標簽抗體 
研究領域 腫瘤  細胞生物  免疫學  染色質和核信號  信號轉導  合成與降解  結合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Recombinant protein
產品應用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Synthetic peptide: HHHHHH conjugated to KLH 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

SWISS:
N/A

Gene ID:
N/A

標簽抗體
蛋白質純化的新途徑─應用His-Tag抗體檢測His-Tag融合蛋白

1） 6xHis與不同的Sepharose純化填料結合（捆綁），可不受構象的影響，在天然或變形的條件下可進行進一步純化。
2） 可在溫和的洗脫液環境中，其結合、洗滌及洗脫步驟不會對蛋白結構產生影響。
3） 6xHis標簽比普通（Tag）要小得多，經純化后的蛋白可直接進行下游操作。可用于任何表達系統（包括：
細菌，桿狀病毒和哺乳動物）
4） 6xHis標簽在生理溶液pH下不帶電荷，其標簽不會干涉重組蛋白的結構和功能， 6xHis不會影響蛋白分泌
5） 6xHis標簽免疫原性差，重組蛋白可以不必除去標簽而直接作為抗原進行免疫
產品圖片
20 ng rGFP-His protein (bs-33009P) per lane probed with His tag polyclonal antibody respectively, unconjugated (bs-0287R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
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